Detection of membrane-bound proteases of Francisella tularensis
| Autor: | A. V. Korneva, V. B. Nikolaev, C. N. Kozlov, E. Y. Markov, A. V. Mazepa, Y. O. Popova |
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| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Acta Biomedica Scientifica, Vol 1, Iss 5, Pp 155-159 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2541-9420 2587-9596 |
| DOI: | 10.12737/23416 |
| Popis: | Sterile preparations of membrane fractions were prepared by processing of live Francisella tularensis cells of different subspecies with 4.5 M urea solution and differential centrifugation. For the first time, proteolytic activity was detected and studied by tests of radial enzyme diffusion and substrate polyacrylamide gel electrophoresis using gelatin as a substrate. Spectrum of gelatinases in the resulted preparation were detected. Quantitative inter-strain differences in the protease activities and their qualitative composition in membrane preparations of various virulent F. tularensis strains was analyzed. Avirulent F. tularensis 21/400 subsp. holarctica (1-214) strain demonstrated the greatest gelatinase activity in enzyme diffusion method and the lowest hydrolytic activity was seen in F. tularensis B-399 A-Cole subsp. tularensis (1-386) and F. tularensis Utah 112 subsp. novicida (1-384), other preparations showed intermediate activity. Enzyme electrophoresis in the protease spectra determined the presence of proteins with proteases activity 50-100 kDa, and in the spectrum preparations of F. tularensis 1-386 and 1-384 were detected additional bands of proteases. |
| Databáze: | Directory of Open Access Journals |
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