| Autor: |
Changliang Yu, Fangqiu Ding, Zhenyu Ma, Yuguo Tang |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Photonics, Vol 11, Iss 11, p 1091 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2304-6732 |
| DOI: |
10.3390/photonics11111091 |
| Popis: |
In clinical practice, microscopes are commonly used for imaging chromosomes to diagnose genetic diseases. Achieving precise and rapid autofocusing is a significant challenge in the advancement of high-throughput chromosome imaging systems. Here, we introduce a rapid autofocus method based on LED oblique illumination for dual-objective configuration in metaphase chromosome imaging system. Our method utilizes a programmable LED array for sample illumination, employing a sequential activation of two LEDs from opposing angles to create oblique illumination. The defocus distance is ascertained through image cross-correlation calculation. Illumination with multiple LEDs in the array is used to acquire bright-field images after completing the focusing. Our method can perform continuous autofocus under a 10× objective and a 100× oil immersion objective, with average focusing errors of 1.29 μm and 0.12 μm, respectively. The total imaging time for a single sample has been significantly reduced from approximately 10 min with conventional methods to just 2 min. This study provides preliminary evidence supporting the viability of developing a next-generation high-throughput chromosome scanner employing a LED array. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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