| Autor: |
Yan Tang, Diego Benusiglio, Arthur Lefevre, Stephanie Küppers, Olga Lapies, Damien Kerspern, Alexandre Charlet, Valery Grinevich |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 1, Pp 101032- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.101032 |
| Popis: |
Summary: Here, we present a step-by-step protocol to target, record, and manipulate the activity of oxytocin neurons in awake rats. The protocol includes a procedure to record the activity of oxytocin neurons from awake and socially interacting rats using opto-electrodes for simultaneous electrophysiological recording and virally based cell-type-specific opto-tagging with Channelrhodopsin 2. Furthermore, we illustrate a procedure for optically guided implantation of optic fiber and imaging of oxytocin neuron population activity expressing calcium indicator GCaMP6s with the fiber photometry technique.For complete details on the use and execution of this protocol, please refer to Tang et al., 2020. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
