A new laboratorial method for the diagnosis of gastrointestinal parasites in dogs

Autor: Willian Marinho Dourado Coelho, Jancarlo Ferreira Gomes, Alessandro Francisco Talamini do Amarante, Katia Denise Saraiva Bresciani, Giuliano Lumina, Sumie Koshino-Shimizu, Denise Pereira Leme, Alexandre Xavier Falcão
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2013
Předmět:
Zdroj: Revista Brasileira de Parasitologia Veterinária, Vol 22, Iss 1, Pp 1-5 (2013)
Druh dokumentu: article
ISSN: 1984-2961
DOI: 10.1590/S1984-29612013000100002
Popis: In this study, we aimed to introduce a new technique calledTF-Test Modified∕Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified∕Dog and the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher in the TF-Test Modified∕Dog (98.41%), followed by flotation in saturated zinc sulfate (77.78%), TF-Test Conventional (73.02%), flotation by saturated sodium chloride (55.55%), and direct microscopy exam (30.16%). The diagnostic efficiency varied from 58.49% to 99.06%, with the highest value also obtained by the new proposed technique. Efficiency level of 99.06% with kappa index 0.979 (almost perfect) was obtained with the TF-Test Modified∕Dog. These results represent significant statistical gains (P < 0.05) of 20.63% in sensitivity and 12.27% in efficiency over the best among the other techniques – flotation by saturated zinc sulfate, whose kappa index was 0.738, much lower than that of the TF-Test Modified∕Dog. All techniques presented 100% specificity. In this sense, the high sensitivity of theTF-Test Modified∕Dog makes it suitable for epidemiological surveys of gastrointestinal parasitosis in dogs, zoonoses control and preventive surveillance programs.
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