Comparative analysis of electro-chemiluminescence immunoassay, enzyme-linked immunosorbent assay and rapid immunochromatographic test for detection of hepatitis B surface antigen

Autor: Mohammad Shahid, Hiba Sami, Parvez Anwar Khan, Sanjay Sharma, Amritesh Kumar Singh, Syed Haider M. Husaini, Haris Manzoor Khan
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: CHRISMED Journal of Health and Research, Vol 10, Iss 1, Pp 110-114 (2023)
Druh dokumentu: article
ISSN: 2348-3334
2348-506X
DOI: 10.4103/cjhr.cjhr_8_21
Popis: Background: Electro-chemiluminescence immunoassay (ECLIA), enzyme-linked immunosorbent assay (ELISA), and rapid immunochromatographic test (RICT) are currently commonly used for the detection of hepatitis B surface antigen (HBsAg). Although a few studies have been performed to compare the efficiency of these three different serological assays, but there are still many grey areas where clear guidelines are required to give the reports as reactive or nonreactive. Materials and Methods: We compared three different methods of detecting HBsAg: RICT, ELISA and ECLIA. The aim of this cross-sectional study was to analyze the performance of these tests and evaluate the cut off index (COI) of ECLIA to be considered as positive. Some of the representative samples that showed a discrepancy between ELISA and ECLIA were confirmed by nucleic acid amplification test (NAAT). Results: A total of 3846 samples were included in the study, of which 259 (6.73%) were positive by ECLIA. Of these 259 samples, 68 were positive by both ECLIA and ELISA and had COI of >5 in ECLIA, whereas 191 were positive by ECLIA only (COI between 0.9 and 5). Hence, the concordance rates of the ECLIA and ELISA in detecting serum HBsAg was 26.25% while the same for ELISA and RICT was 31.57%. Four representative samples which were positive by ECLIA and ELISA (i.e., COI >5) were positive with NAAT. However, one sample which was negative by ELISA and positive by ECLIA (i.e., COI
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