Antibacterial activity of camel colostrum against pathogenic strain of Escherichia coli F17-associated with calf diarrhea
| Autor: | Salma Bessalah, Asim Faraz, Ayman Balla Mustafa, Syeda Maryam Hussain, Shamsaldeen Ibrahim Saeed, Chanda Liaqat, Waqas Ashraf, Zeeshan Muhammad Iqbal, Muhammad Arslan Akbar, Mohamed Hammadi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Open Veterinary Journal, Vol 14, Iss 11, Pp 2883-2892 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2226-4485 2218-6050 |
| DOI: | 10.5455/OVJ.2024.v14.i11.17 |
| Popis: | Background: Many protective proteins, including lactoferrin and heavy chain antibodies, are present in camel colostrum, giving it a distinctive composition. Beyond a broad spectrum of pathogens, these proteins demonstrate antibacterial properties. Aim: The current research assessed the prophylactic properties of camel colostrum against Escherichia coli F17. Methods: A microbroth dilution method was employed to assess the efficacy of camel colostrum, whereas a crystal violet assay was utilised to determine its antibiofilm potential. Extracellular deoxyribonuclease acid (eDNA) release, swarming, and swimming motilities were also examined. Results: The results showed that camel colostrum significantly reduced E. coli-F17 growth by 70% and above at different incubation periods (6 - 24h). The rate of cell attachment gradually decreased from approximately 40% to 24% as the concentration increased from 12.5 to 50 mg/mL. E. coli-F17 developed a biofilm at a rate of 54.8% when exposed to 50 mg/mL of camel colostrum. In contrast, the greatest level of biofilm formation against the tested bacteria (94%) was observed at a concentration of 1.5 mg/mL. A halo zone of camel colostrum ranging from 10 to less than 30 mm at concentrations between 6 and 50 mg/mL also inhibited swimming and swarming capabilities. The treated cells yielded no eDNA. Conclusion: According to these results, camel colostrum inhibits the growth of E. coli-F17 by impeding the swarming and swimming motilities, and biofilm formation. Additionally, camel colostrum incubation with E. coli-F17 diminishes eDNA. To evaluate the potential protective effects of camel colostrum in an animal model, additional research is recommended. [Open Vet J 2024; 14(11.000): 2883-2892] |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|