Cyclopamine, an Antagonist of Hedgehog (Hh) Signaling Pathway, Reduces the Hatching Rate of Parthenogenetic Murine Embryos
| Autor: | Jaehyun Park, Jeonghyeon Moon, So Min, Stephan Chae, Sangho Roh |
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| Jazyk: | English<br />Korean |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Journal of Animal Reproduction and Biotechnology, Vol 33, Iss 4, Pp 237-243 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2671-4639 2671-4663 |
| DOI: | 10.12750/JET.2018.33.4.237 |
| Popis: | Hedgehog (Hh) pathway plays a key role in development from invertebrate to vertebrate. It is known to be involved in cell differentiation, polarity, proliferation, including the development of vertebrate limb and the establishment of flies’ body plan. To investigate how the regulation of Hh pathway affects the development of parthenogenetic murine embryos, the parthenogenetically activated murine embryos were treated with either cyclopamine (Cyc), an antagonist of Hh pathway, or purmorphamine, an agonist of Hh pathway. While Cyc did not affect the blastocyst formation and its total cell number, the chemical reduced the hatching rate of embryos and the expression levels of Fn1 mRNA. The results of the present study show the possibility that Cyc may affect the development of embryos at blastocyst stage by blocking Hh pathway and this may cause detrimental effect to the embryos at peri-, and post-implantation stages. |
| Databáze: | Directory of Open Access Journals |
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