Desenvolvimento e validação de um método analítico rápido por cromatografia líquida de alta eficiência para determinação de nimesulida em estudos de liberação in vitro Development and validation of a rapid analytical method by HPLC for determination of nimesulide in release studies
| Autor: | André Luís Morais Ruela, Magali Benjamim Araújo, Gislaine Ribeiro Pereira |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 32, Iss 1, Pp 165-168 (2009) |
| Druh dokumentu: | article |
| ISSN: | 0100-4042 1678-7064 |
| DOI: | 10.1590/S0100-40422009000100031 |
| Popis: | A high performance liquid chromatography (HPLC) method has been developed for a rapid determination of nimesulide in dissolution studies. Nimesulide was analyzed using 5 µm Lichrospher® RP-18 column (125 x 4 mm i.d.) and mobile phase acetonitrile: phosphate buffer pH=6.0 (55:45) at a flow-rate of 1.0 mL min-1. Detection was carried out at 300 nm at 25 ºC. The method was applied to analysis of nimesulide in in vitro release studies and showed a rapid and efficient analytical alternative for evaluation of dissolution profile of nimesulide. |
| Databáze: | Directory of Open Access Journals |
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