| Autor: |
Feoktisova Natalia A., Nafeev Alexander A., Mastilenko Andrey V., Bogdanov Ilgizar I., Lomakin Artyom A., Minaeva Angelina N., Rodionova Arina V. |
| Jazyk: |
English<br />French |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
BIO Web of Conferences, Vol 71, p 01076 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2117-4458 |
| DOI: |
10.1051/bioconf/20237101076 |
| Popis: |
As a result of the experiments, the parameters for setting up a polymerase chain reaction were developed for the accelerated identification of Aeromonas spp. The design of species-specific primers was performed, the reaction parameters were selected, the specificity was checked, and the optimal amplification time was selected. As a result of this study, a multiplex real-time PCR test system was developed for the simultaneous detection of A. salmonicida, A. caviae and A. veronii. The sensitivity of the developed protocol was 5.56 pg/µl. The data obtained will allow for accelerated screening of these pathogens. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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