| Autor: |
Masayuki Uchida, Yukio Ohshiba, Orie Mogami |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Journal of Pharmacological Sciences, Vol 117, Iss 1, Pp 63-66 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1347-8613 |
| DOI: |
10.1254/jphs.11089SC |
| Popis: |
Abstract.: Trypsin-treated β-lactoglobulin significantly decreased the glucose level after an oral glucose tolerance test using mice. We performed the present study to identify the active peptide inhibiting dipeptidyl peptidase-4 from trypsin-treated β-lactoglobulin. Trypsin-treated β-lactoglobulin showed a concentration-dependent inhibition for dipeptidyl peptidase-4, with an IC50 value of 210 μM, although non-treated β-lactoglobulin showed no significant effect in the in vitro assay. The active peptide was isolated from trypsin-treated β-lactoglobulin and identified as the hexapeptide Val-Ala-Gly-Thr-Trp-Tyr (β-lactoglobulin f15-20). This hexapeptide also exhibited a concentration-dependent inhibitory effect and IC50 value was 174 μM, suggesting that this hexapeptide is almost totally responsible for the DPP-4 inhibitory activity of trypsin-treated β-lactoglobulin. Keywords:: DPP-4 inhibitor, β-lactoglobulin, Val-Ala-Gly-Thr-Trp-Tyr |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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