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ObjectiveTo study the intervention effect and mechanism of Zhuanggu Jianxi decoction on synovitis in knee osteoarthritis (KOA) rabbits via the inflammatory regulation of synovial tissue and the expression of key node proteins in LXRs/NF-κB pathway.MethodsBlank serum and drug-containing serum of rabbits were prepared for the intervention of synovial tissue. Twenty-two rabbits were randomly divided into two groups, with 16 in the model group and 6 in the blank group. The KOA model was injected with papain into the knee joint of the right hind limb. After the model was identified successfully, the synovial tissue of the right hind limb knee was cut into small pieces with a size about 1-2 mm3 under aseptic operation, 60 pieces were collected and randomly divided into model group, Zhuanggu Jianxi decoction group, LXRα inhibitor group and N-CoR inhibitor group with 15 pieces each, the synovial tissue pieces in each group were cultured in 6 well plates with every 3 pieces in each well, the four groups were intervened with 10% blank serum, 10% drug-containing serum, 10% drug-containing serum+5CPPSS-50 and 10% drug-containing serum+ML-792 respectively; the synovium of the blank group was prepared by the same method and cultured with 10% blank serum for 5 wells. After 7 days, synovial tissue was collected. Two wells of synovial tissue were taken from each group for HE staining, and the remaining synovial tissue was detected by Western blot and ELISA.Results1) Morphology: compared with the blank group, HE staining in the model group showed the proliferation and disorder of synovial lining cells, a large number of inflammatory cell infiltration and fibrous tissue hyperplasia, and the morphological score was significantly increased (PPPPPPPPPP>0.05), P50 and P65 protein expression were significantly decreased (PPPPP |
