Electron Microscopy for Rapid Diagnosis of Emerging Infectious Agents

Autor:	Paul R. Hazelton, Hans R. Gelderblom
Jazyk:	angličtina
Rok vydání:	2003
Předmět:	Rapid diagnostic electron microscopy morphological diagnosis negative staining particle enrichment bioterrorism agri-terrorism Medicine Infectious and parasitic diseases RC109-216
Zdroj:	Emerging Infectious Diseases, Vol 9, Iss 3, Pp 294-303 (2003)
Druh dokumentu:	article
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid0903.020327
Popis:	Diagnostic electron microscopy has two advantages over enzyme-linked immunosorbent assay and nucleic acid amplification tests. After a simple and fast negative stain preparation, the undirected, “open view” of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen. Details for efficient sample collection, preparation, and particle enrichment are given. Applications of diagnostic electron microscopy in clinically or epidemiologically critical situations as well as in bioterrorist events are discussed. Electron microscopy can be applied to many body samples and can also hasten routine cell culture diagnosis. To exploit the potential of diagnostic electron microscopy fully, it should be quality controlled, applied as a frontline method, and be coordinated and run in parallel with other diagnostic techniques.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/5d86105a892741aa9435c87899d50bd8 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF