Downregulation of Calcineurin Gene Is Associated with Glucantime(®) Resiatance in Leishmania infantum
| Autor: | Mohammad Bagher Khadem Erfan, Mehdi Mohebali, Elham Kazemi-Rad, Homa Hajjaran, Gholamhossein Edrissian, Setareh Mamishi, Mojtaba Saffari, Reza Raoofian, Mansour Heidari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Iranian Journal of Parasitology, Vol 8, Iss 3 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1735-7020 2008-238X |
| Popis: | Background: Pentavalent antimonials are the first line drugs for the treatment of leishmaniasis. Unresponsiveness of Leishmania spp. to antimonial drugs is a serious problem in some endemic areas. Investigations on molecular mechanisms involved in drug resistance are essential for monitoring and managing of the disease. Cal-cineurin is an essential protein phosphatase for number of signal transduction pathways in eukaryotic cells and it has a mediated role in apoptosis. This study aimed to determine of biomarker(s) in Glucantime® resiatance strain of L. infan-tum. Methods: We used cDNA amplified fragment length polymorphism (cDNA-AFLP) and real time-RT PCR assays to compare gene expression profiles at the mRNA levels in resistant and susceptible L. infantum field isolates. Results: The cDNA-AFLP results showed downlegulation of calcineurin in resis-tant isolate in comparison with susceptible one. Significant downregulation of cal-cineurin (0.42 fold) (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|