Effect of elevated irrigation bottle height during cataract surgery on corneal endothelial cells in porcine eyes

Autor: Daniel A. Wenzel, Constanze Schultheiss, Vasyl Druchkiv, Olaf J. C. Hellwinkel, Martin S. Spitzer, Maximilian Schultheiss, Maria Casagrande, Nils Alexander Steinhorst
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: BMC Ophthalmology, Vol 23, Iss 1, Pp 1-9 (2023)
Druh dokumentu: article
ISSN: 1471-2415
DOI: 10.1186/s12886-023-02954-w
Popis: Abstract Background Cataract surgery induces corneal endothelial cell loss (ECL). This study investigates the relationship between bottle height (BH) and ECL induced due to irrigation and aspiration (I/A) in cataract surgery and quantifies protective effects of intraoperatively used ophthalmic viscoelastic substances. Methods Intermittent I/A without phacoemulsification was performed in porcine eyes for 10 min with varying BHs of 100 cm (BH100), 125 cm (BH125), 150 cm (BH150) or no treatment (control, no I/A). Additionally, in one group a dispersive ophthalmic viscoelastic substance was injected into the anterior eye chamber before treatment with I/A at a BH of 150 cm (BH150 + V). After exposure of the corneal endothelium to I/A, the corneas were prepared to split corneal buttons on day 0 and cultivated for 15 days. Endothelial cell density (ECD) was analyzed blinded on days 1, 8 and 15. Results Relative ECL significantly correlated with irrigation BH (control (n = 13): -9.69 ± 6.03% (average ± standard deviation); BH100 (n = 12): -9.69 ± 4.81%—p = 1.000; BH125 (n = 14): -19.44 ± 7.30% – p
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