Bioflocculant Technology Implementation Using Navicula sp for Harvesting of Arthrospira Platensis Culture
| Autor: | Dr. Eko Agus Suyono, Jody Ashrib Satriayudistira, Yahya Baihaqi, Ria Amelia |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Squalen, Vol 19, Iss 2, Pp 97-106 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2089-5690 2406-9272 |
| DOI: | 10.15578/squalen.883 |
| Popis: | Bioflocculation, a microalgae harvesting method utilizing organisms like bacteria and microalgae as flocculant agents, necessitates careful selection and ratio determination of bioflocculant agents. This study seeks to ascertain the optimal balance of bioflocculant, Navicula sp., for metabolite production and harvesting efficiency in Arthrospira platensis culture. Spanning three oneweek cultivation stages, the research commences with A. platensis culture under varying salinities (0, 15, 25, and 35 ppt) in the first stage, followed by pH variations (9, 10, 11, and 12) in the second stage. The optimal salinity-pH combination identified in the first two stages is applied in the third stage, involving bioflocculant addition to culture and medium solutions at varying ratios (0.25:1, 0.5:1, 0.75:1, and 1:1 v/v). Precipitation efficiency and pigmentation were measured using spectrophotometry, while lipid content was determined by the Bligh Dyer extraction method. Carbohydrate content was assessed using the Phenol-Sulphuric Acid assay, and protein content was quantified using the Bradford protein assay. The findings indicate salinity, pH, and bioflocculant influence A. platensis growth, metabolite production, and harvesting efficiency. Optimal outcomes are achieved at 15 ppt salinity, pH 9, and a 1:1 ratio of bioflocculant, yielding 1.18x10-3 g of dry biomass, 3.56x105 cells/mL of cell density, 2.9x100 g/L of carbohydrates, 3.63x100 g/L of lipids, 9.97x10-3 mg/L of chlorophyll a+b, 8.71x10-1 mg/L of carotenoids, and 3.04x10-1 g/L of protein. These results suggest that salinity addition, high pH, and the addition of Navicula sp. as bioflocculant increase the harvesting process efficiency of A. platensis culture. |
| Databáze: | Directory of Open Access Journals |
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