| Autor: |
Kuo-Chin Lo, Jualang Azlan Gansau, Chia-Hung Shih, Chien-Yuan Kao |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Horticulturae, Vol 8, Iss 3, p 206 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2311-7524 |
| DOI: |
10.3390/horticulturae8030206 |
| Popis: |
This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 to 8 h before sectioning with a microtome. Optimal conditions for slicing sections of 100 to 300 μm were achieved when the protocorms were embedded at 10% (w/v) agarose and dried for 4 h under laminar flow, and the hardness of the agarose block under these conditions reached 641.8 ± 9.5 g·cm−2. The sectioned protocorms that were cultured on an MS medium supplemented with 1.2 mg·L−1 6-benzylaminopurine and 0.1 mg·L−1 α-naphthaleneacetic acid were capable of growth and differentiated through the neoformation of protocorm-like bodies (PLBs) and/or callus before subsequent regeneration into plantlets and development into healthy plants in a nursery environment. The results of this study demonstrate that microtome-based tTCL is a reliable and promising approach for mass propagation and possible virus-free propagation objectives for Phalaenopsis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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