| Autor: |
Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, László G. Puskás, Gabriella Fábián, Liliána Z. Fehér, Pradeep Kumar Reddy Cingaram, Bhavyashree Suresh, Begüm Peksel, Soujanya Kuntam, Eszter Molnár |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Molecules, Vol 18, Iss 8, Pp 9999-10013 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
1420-3049 |
| DOI: |
10.3390/molecules18089999 |
| Popis: |
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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