| Autor: |
Nararatn Wattanapan, Charassri Nualsri, Upatham Meesawat |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Songklanakarin Journal of Science and Technology (SJST), Vol 40, Iss 2, Pp 306-313 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
0125-3395 |
| DOI: |
10.14456/sjst-psu.2018.48 |
| Popis: |
Paphiopedilum callosum var. sublaeve, a lady’s slipper orchid native to southern Thailand, is protected under CITES (appendix I) due to habitat destruction and over-collection. The novel micropropagation technique for this endemic species was investigated. A 2-week preculture of seeds in distilled water before transfer to 1/2MS solid medium was the optimal conditions to promote seed germination in vitro. Shoot tip-derived transverse thin cell layer (tTCL) explants cultured on MVW solid medium containing 1.0 mg/L TDZ for 8 weeks provided the highest percentage of regenerated protocorm-like bodies (46.67±6.67), shoot formation (40.00±5.16), root formation (30.00±12.38) and survival rate (70.00±4.47). The acclimatized plantlets potted in sphagnum moss in the greenhouse grew well at 80% survival rate. No genetic variation was detected between the regenerated plantlets and their own mother plants based on RAPD marker. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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