IDENTIFICATION OF DIFFERENTLY METYLATED GENES POTENTIALLY RELATED TO HUMAN ATHEROSCLEROSIS
| Autor: | M. S. Nazarenko, А. V. Markov, Yu. A. Koroleva, А. A. Sleptsov, А. N. Kazantsev, О. L. Barbarash, V. P. Puzyrev |
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| Jazyk: | ruština |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Российский кардиологический журнал, Vol 0, Iss 10, Pp 42-48 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1560-4071 2618-7620 |
| DOI: | 10.15829/1560-4071-2017-10-42-48 |
| Popis: | Aim. Identification of the genes, different in the level of DNA methylation among the cells of intact or atherosclerotic arteries in patients with coronary and carotid atherosclerosis.Material and methods. Into the study group, atherosclerosis patients were included, who had undergone coronary bypass surgery or carotid endarterctomy, and relatively healthy individuals. The assessment of methylation level of various 27578 CpG-sites/14475 genes was done with the microchip Infinium Human Methylation27 BeadChip (Illumina) in the specimens of atherosclerotically changed coronary (n=6), carotid (n=6), intact internal thoracal arteries (n=8) and large saphenous veins (n=8). Level of methylation in the locus 2q31.1 (HOXD4/HOXD3/MIR10B) was measured with bisulphite pyrosequencing of DNA in the vessels specimens and leucocytes of the same patients (n=130), as in the leucocytes of relatively healthy men (n=110).Results. In the cells of atherosclerotically changed arteries, comparing to intact vessels, the change of methylation level by 20% and more is found for 46 CpG-sites/42 genes (pFDR |
| Databáze: | Directory of Open Access Journals |
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