Hydro-alcoholic Extraction by an Ultrasound Assisted Leaching Method of Crotalaria assamica Benth Seed Powder for Pharmaceutical Purposes: Optimization using RSM and Basic Properties

Autor: Long Hoang Nguyen, Viet Tien Vo, Minh Thong Le Tuan, Tan Phat Vo, Nhan Tu Le Tan, Dinh Quan Nguyen
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Chemical Engineering Transactions, Vol 97 (2022)
Druh dokumentu: article
ISSN: 2283-9216
DOI: 10.3303/CET2297018
Popis: Many components in Crotalaria assamica benth composition make this plant a potential antioxidant and antibacterial herbal material, which can find many applications in medicine and biochemistry. In this study, Crotalaria assamica benth seed powder was extracted with aqueous alcohol solutions by an ultrasound-assisted leaching method. The ultrasound wave was 37 kHz, and the extraction was carried out at 30 °C for 10 mins. An alcohol content of 54.78 vol% and a mass ratio of solid to liquid (RSL) of 1/55 are suitable conditions according to the reaction surface method (RSM) developed by the Box–Beknen approach to design matrix experiments. With the above conditions, the actual result was that the TPC was 12.02 ± 0.24 mg GAE/g DW and the TFC was 7.68 ± 0.34 mg RE/g DW). Crotalaria assamica confers antioxidant activity of the extract with an IC50 value of 644 ??g/mL, and antibacterial activity against P. aeruginosa strain, with an average antibacterial ring diameter of 13.5 mm.
Databáze: Directory of Open Access Journals