Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

Autor:	Sarah Gutch, Lindsay Beasley, Scott Cooper, Mark H. Kaplan, Maegan L. Capitano, James Ropa
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	Cell culture Cell isolation Stem Cells Cell Differentiation Science (General) Q1-390
Zdroj:	STAR Protocols, Vol 5, Iss 2, Pp 103024- (2024)
Druh dokumentu:	article
ISSN:	2666-1667 17057205
DOI:	10.1016/j.xpro.2024.103024
Popis:	Summary: Umbilical cord blood (CB) is a donor source for hematopoietic cell therapies. Understanding what drives hematopoietic stem and progenitor cell function is critical to our understanding of the usage of CB in hematopoietic cell therapies. Here, we describe how to isolate and analyze the function of human hematopoietic cells from umbilical CB. This protocol demonstrates assays that measure phenotypic properties and hematopoietic cell potency.For complete details on the use and execution of this protocol, please refer to Broxmeyer et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/5cab2e396aa74324a0eada7b17057205 Zobrazit plný text záznamu View record in DOAJ