| Autor: |
Fan Zhong, Xiao-Zhen Ding, Wei-Zhong Yang, Zong-Yin Gao, Xiao-He Lu |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Guoji Yanke Zazhi, Vol 15, Iss 7, Pp 1134-1138 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1672-5123 |
| DOI: |
10.3980/j.issn.1672-5123.2015.7.04 |
| Popis: |
AIM: To explore the inhibiting effect of FTY720 on corneal neovascularization(CNV)of rat.METHODS: MTT assay and cells scratch were adopted to observe hyperplasia of human umbilical vein endothelial cells(HUVECs)and cell migration induced by sphingosine-1-phosphate(S1P)after using FTY720 of different concentration. The effect of FTY720 on CNV induced by S1P in a rat corneal micropocket model was detected. 30SD rats were randomly divided into group A, group B and group C with 10 rats per group. S1P and 0μg, 5μg, and 20μg FTY720 controlled-released particles were implanted into the corneal stroma. The growth of CNV and having pathological examination on 12d after the operation was observed. Findings was analyzed by one-way ANOVA.RESULTS: 10, 102, 103, and 104 nmol/L FTY720 and HUVECs co-incubate 72h could inhibit cell proliferation(P2(F=145.155, PPCONCLUSION: FTY720 can inhibit S1P-induced corneal neovascularization. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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