CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
| Autor: | Hiroyuki Morisaka, Kazuto Yoshimi, Yuya Okuzaki, Peter Gee, Yayoi Kunihiro, Ekasit Sonpho, Huaigeng Xu, Noriko Sasakawa, Yuki Naito, Shinichiro Nakada, Takashi Yamamoto, Shigetoshi Sano, Akitsu Hotta, Junji Takeda, Tomoji Mashimo |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2041-1723 35936827 |
| DOI: | 10.1038/s41467-019-13226-x |
| Popis: | Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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