Single‐Cell Transcriptome Landscape and Cell Fate Decoding in Human Brain Organoids after Transplantation

Autor: Shi‐Bo Xu, Xin‐Rui Li, Pan Fan, Xiyang Li, Yuan Hong, Xiao Han, Shanshan Wu, Chu Chu, Yuejun Chen, Min Xu, Mingyan Lin, Xing Guo, Yan Liu
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Advanced Science, Vol 11, Iss 28, Pp n/a-n/a (2024)
Druh dokumentu: article
ISSN: 2198-3844
20240228
DOI: 10.1002/advs.202402287
Popis: Abstract Human stem cells and derivatives transplantation are widely used to treat nervous system diseases, while the fate determination of transplanted cells is not well elucidated. To explore cell fate changes of human brain organoids before and after transplantation, human brain organoids are transplanted into prefrontal cortex (PFC) and hippocampus (HIP), respectively. Single‐cell sequencing is then performed. According to time‐series sample comparison, transplanted cells mainly undergo neural development at 2 months post‐transplantation (MPT) and then glial development at 4MPT, respectively. A different brain region sample comparison shows that organoids grafted to PFC have obtained cell fate close to those of host cells in PFC, other than HIP, which may be regulated by the abundant expression of dopamine (DA) and acetylcholine (Ach) in PFC. Meanwhile, morphological complexity of human astrocyte grafts is greater in PFC than in HIP. DA and Ach both activate the calcium activity and increase morphological complexity of astrocytes in vitro. This study demonstrates that human brain organoids receive host niche factor regulation after transplantation, resulting in the alignment of grafted cell fate with implanted brain regions, which may contribute to a better understanding of cell transplantation and regenerative medicine.
Databáze: Directory of Open Access Journals
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