| Autor: |
Hyueyun Kim, Ji Ha Choi, Chang Mo Moon, Jihee Lee Kang, Minna Woo, Minsuk Kim |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biological Procedures Online, Vol 24, Iss 1, Pp 1-12 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1480-9222 |
| DOI: |
10.1186/s12575-022-00178-8 |
| Popis: |
Abstract Background Micro RNA of Marsupenaeus japonicas has been known to promote apoptosis of tumor cells. However, the detailed mechanisms are not well understood. Results Using tomographic microscope, which can detect the internal structure of cells, we observed breast tumor cells following treatment of the miRNA. Intriguingly, we found that mitochondria migrate to an adjacent tumor cells through a tunneling nanotube. To recapitulate this process, we engineered a microfluidic device through which mitochondria were transferred. We show that this mitochondrial transfer process released endonuclease G (Endo G) into tumor cells, which we referred to herein as unsealed mitochondria. Importantly, Endo G depleted mitochondria alone did not have tumoricidal effects. Moreover, unsealed mitochondria had synergistic apoptotic effects with subtoxic dose of doxorubicin thereby mitigating cardiotoxicity. Conclusions Together, we show that the mitochondrial transfer through microfluidics can provide potential novel strategies towards tumor cell death. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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