Cumulative expression of heterologous XlnR regulatory modules and AraRA731V in Penicillium oxalicum enhances saccharification efficiency of corn stover and corn fiber

Autor: Chengqiang Xia, Xiaoyu Qi, Xin Song
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Biotechnology for Biofuels and Bioproducts, Vol 17, Iss 1, Pp 1-12 (2024)
Druh dokumentu: article
ISSN: 2731-3654
DOI: 10.1186/s13068-024-02464-x
Popis: Abstract Penicillium oxalicum engineered strain DB2 and its mutant strains with multiple regulatory modules were constructed. Mutant strain RE-4-2 with two regulatory modules showed a significant increase in the reducing sugar released from corn stover and corn fiber as well as in the conversion of cellulose than DB2. RE-5-2 with three regulatory modules showed a further increase in reducing sugar released from corn stover and the conversion of cellulose on the basis of RE-4-2. RE-4-2-AraRA731V constructed by overexpressing AraRA731V in RE-4-2 showed an increase of 7.2 times and 1.2 times in arabinofuranosidase and xylosidase activities, respectively. Reducing sugar yield and cellulose conversion of corn stover and corn fiber by RE-4-2-AraRA731V were further increased.
Databáze: Directory of Open Access Journals
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