Impact of surface wettability on S-layer recrystallization: a real-time characterization by QCM-D

Autor: Jagoba Iturri, Ana C. Vianna, Alberto Moreno-Cencerrado, Dietmar Pum, Uwe B. Sleytr, José Luis Toca-Herrera
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Beilstein Journal of Nanotechnology, Vol 8, Iss 1, Pp 91-98 (2017)
Druh dokumentu: article
ISSN: 2190-4286
DOI: 10.3762/bjnano.8.10
Popis: Quartz crystal microbalance with dissipation monitoring (QCM-D) has been employed to study the assembly and recrystallization kinetics of isolated SbpA bacterial surface proteins onto silicon dioxide substrates of different surface wettability. Surface modification by UV/ozone oxidation or by vapor deposition of 1H,1H,2H,2H-perfluorododecyltrichlorosilane yielded hydrophilic or hydrophobic samples, respectively. Time evolution of frequency and dissipation factors, either individually or combined as the so-called Df plots, showed a much faster formation of crystalline coatings for hydrophobic samples, characterized by a phase-transition peak at around the 70% of the total mass adsorbed. This behavior has been proven to mimic, both in terms of kinetics and film assembly steps, the recrystallization taking place on an underlying secondary cell-wall polymer (SCWP) as found in bacteria. Complementary atomic force microscopy (AFM) experiments corroborate these findings and reveal the impact on the final structure achieved.
Databáze: Directory of Open Access Journals
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