| Autor: |
Mukesh Kumar Patidar, Anand Nighojkar, Sadhana Nighojkar, Anil Kumar |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Canadian Journal of Biotechnology, Vol 1, Iss 1, Pp 11-18 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2560-8304 |
| DOI: |
10.24870/cjb.2017-000102 |
| Popis: |
Polygalacturonase, an industrial enzyme has been produced from various fungal isolates using solid state and submerged fermentation techniques. The challenge has been yield, extraction and cost of production. In the present study, a low cost solid substrate, dried papaya peel was employed for polygalacturonase production using Aspergillus niger AN07. Polygalacturonase enzyme from Aspergillus niger AN07 was purified to 24.8 fold with a 52.6% recovery through anion exchange chromatography on DEAE-cellulose and gel filtration chromatography using Sephadex G-200. The SDS-PAGE revealed that the enzyme was monomeric with a molecular weight of 64.5 kDa. The optimum pH and temperature were 5.0 and 55℃, respectively. This enzyme was stable over a wide pH range (4.0-7.0) and relatively high temperature of 55℃ for 1 h. The Km and Vmax values of polygalacturonase for polygalacturonic acid were 2.6 mg/l and 181.8 µmol/ml/min, respectively. The purified enzyme could digest the polygalacturonic acid into oligosaccharides with a small amount of galacturonic acid as visualized on thin layer chromatography. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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