Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs

Autor: Xianlu Lei, Liguang Xu, Shanshan Song, Liqiang Liu, Hua Kuang
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Food and Agricultural Immunology, Vol 29, Iss 1, Pp 254-266 (2018)
Druh dokumentu: article
ISSN: 0954-0105
1465-3443
09540105
DOI: 10.1080/09540105.2017.1371114
Popis: An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples. The ic-ELISA, with optimized pH, methanol content and sodium chloride content, exhibited an IC50 value of 0.2 ng/mL for FF and 0.27 ng/mL for TAP, with the working range of 0.05–0.77 and 0.05–1.42 ng/mL, respectively. The optimized ic-ELISA showed negligible cross-reactivity with other phenols and broad-spectrum antibiotics. The recoveries in egg samples using the ic-ELISA ranged from 84% to 115% with a coefficient of variation of less than 5%. Based on this monoclonal antibody, a rapid and ultrasensitive immunochromatographic strip assay was developed with a cutoff value of 1 ng/mL for FF and TAP. Our results indicated that both developed methods were highly useful for screening FF and TAP in eggs.
Databáze: Directory of Open Access Journals
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