Metabolomic profiling of pheochromocytomas in dogs: Catecholamine phenotype and tricarboxylic acid cycle metabolites

Autor: Marit F. van denBerg, Nicole Bechmann, Hans S. Kooistra, Monique E. vanWolferen, Elpetra P. M. Timmermans‐Sprang, Mirko Peitzsch, Sara Galac
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Veterinary Internal Medicine, Vol 38, Iss 5, Pp 2415-2424 (2024)
Druh dokumentu: article
ISSN: 1939-1676
0891-6640
DOI: 10.1111/jvim.17148
Popis: Abstract Background In humans with pheochromocytomas (PCCs), targeted metabolomics is used to determine the catecholamine phenotype or to uncover underlying pathogenic variants in tricarboxylic acid (TCA) cycle genes such as succinate dehydrogenase subunits (SDHx). Hypothesis/Objectives To analyze catecholamine contents and TCA cycle metabolites of PCCs and normal adrenals (NAs). Animals Ten healthy dogs, 21 dogs with PCC. Methods Prospective observational study. Dogs diagnosed with PCC based on histopathological and immunohistochemical confirmation were included. Tissue catecholamine contents and TCA metabolites in PCCs and NAs were measured by liquid chromatography with mass spectrometry or electrochemical detection. Results Compared to NAs, PCCs had significantly higher tissue proportion of norepinephrine (88% [median: range, 38%‐98%] vs 14% [11%‐26%]; P
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