| Autor: |
Xiaona Xu, Shuangshuang Ping, Fen Wang, Muhammad Saleem Chang, Guangtong Song |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Aquaculture Reports, Vol 36, Iss , Pp 102077- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2352-5134 |
| DOI: |
10.1016/j.aqrep.2024.102077 |
| Popis: |
Folic acid (FA) is an essential vitamin for the growth and health of aquatic organisms in the aquaculture industry. The purpose of this study was to investigate the effects and potential mechanisms of action of dietary FA on the growth and immune response of red swamp crayfish (Procambius clarkii). Crayfish (n = 360; 3 tanks of 20 crayfish for each dietary treatment) were fed diets containing different levels of FA (0 [control], 0.5, 1, 2, 4, and 8 mg/kg dry feed weight) for 60 d. Broken-line analysis based on the final weight and specific growth rate revealed that the optimal dietary FA level for promoting growth was 2 mg/kg. Villus length, lipase activity, and antioxidant superoxide dismutase activity significantly increased under the 2 mg/kg treatment. Transcriptome analysis identified 177 upregulated and 298 downregulated differentially expressed genes (DEGs) between the 0 (control) and 2 mg/kg groups. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that the most significantly enriched pathways were related to glucose metabolism. Among the DEGs involved in glucose metabolism, Tpi1 (triosephosphate isomerase 1) and Pck1 (phosphoenolpyruvate carboxykinase 1) were upregulated and downregulated under FA treatment, respectively. Our findings suggest that the optimal dietary FA requirement for crayfish is 2 mg/kg and that dietary FA may enhance nutrient bioavailability and regulate glucose metabolism to improve crayfish growth performance. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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