A quantitative sequencing framework for absolute abundance measurements of mucosal and lumenal microbial communities

Autor: Jacob T. Barlow, Said R. Bogatyrev, Rustem F. Ismagilov
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-020-16224-6
Popis: Changes in microbial taxa are commonly derived from estimations of relative abundance, which inherently limits the depth of the analysis. Here, the authors present an absolute quantification method based on digital PCR anchoring and 16S rRNA gene sequencing and show how a ketogenic diet affects individual taxon’s absolute abundance in stool and small-intestine mucosa samples in mice
Databáze: Directory of Open Access Journals