| Autor: |
Achmad Fuad Hafid, Chie Aoki-Utsubo, Adita Ayu Permanasari, Myrna Adianti, Lydia Tumewu, Aty Widyawaruyanti, Sri Puji Astuti Wahyuningsih, Tutik Sri Wahyuni, Maria Inge Lusida, Soetjipto, Hak Hotta |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 7, Pp 633-639 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2221-1691 |
| DOI: |
10.1016/j.apjtb.2017.06.003 |
| Popis: |
Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC50) value of (1.5 ± 0.6) μg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC50 values being (6.5 ± 0.3) μg/mL and (9.7 ± 1.1) μg/mL, respectively. A time-of-addition studies showed that DCM extract from A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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