HPV genotype distribution among women with normal and abnormal cervical cytology presenting in a tertiary gynecology referral Clinic in Ethiopia

Autor: Dawit Wolday, Muluken Derese, Solomon Gebressellassie, Bekure Tsegaye, Wondwossen Ergete, Yirgu Gebrehiwot, Orit Caplan, Dana G. Wolf, Shlomo Maayan
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Infectious Agents and Cancer, Vol 13, Iss 1, Pp 1-8 (2018)
Druh dokumentu: article
ISSN: 1750-9378
DOI: 10.1186/s13027-018-0201-x
Popis: Abstract Background Cervical cancer is the second most prevalent cancer among women of child-bearing age in Ethiopia. The aim of this study was to determine human papilloma virus (HPV) genotype distribution among HIV-negative women with normal and abnormal cervical cytology results. Methods We investigated a consecutive of 233 HIV-negative women between December 2008 and March 2009 presenting in a Tertiary Gynecology Referral Clinic in Ethiopia. Screening was done by Pap cytology and HPV detection and genotyping method was nested PCR (direct amplification with MY09/MY11 primers, followed by nested amplification with GP5/GP6 primers) and sequencing of the nested products. Sequencing of the non-purified nested PCR products was performed following re-amplification with Big dye terminator, using the GP6 primer. Results Of the 233 study participants, 92 (39.5%) had abnormal cytology. All women with abnormal cervical cytology had positive HPV DNA compared to only 48.9% of those presenting with normal cytology. Of these women, the frequency of high-risk (HR)-HPV was 83.2% and its prevalence in women with abnormal cervical cytology was significantly higher than those with normal cytology (92.4% vs. 71%, p
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje