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Today, Salmonella enterica subspecies enterica serovar Enteritidis (S. Enteritidis) represents one of the most common serotypes that causes enterocolitis. Since S. enteritidis identification methods are advanced permanently, the following phenotyping methods could be applied for this purpose: biotyping, phagotyping (phage typing – PT), and resistotyping. From methods for genotyping of S. Enteritidis, plasmid profile analysis (PP), restriction analysis of the virulence plasmid, ribotyping, pulsed field gel electrophoresis (PFGE), insertion sequences, polymerase chain reaction (PCR), random amplified polymorphic DNA analysis (RAPD) could be applied. On the one hand, S. Enteritidis expresses clearly homogenous structure which is reflected by domination of few phagotypes, presence of one plasmid profile in most of strains, merely three clonal lines, as well as a large number of electrophoretic types in a single dendrogram line. Insufficient discrimination of typing systems of S. Enteritidis suggests the introduction of new typing methods as well as improvement of the old ones. |
