Preparation and Characterization of High Purity Anhydrous β-Lactose from α-Lactose Monohydrate at Mild Temperature

Autor: Ana L. López-Pablos, César C. Leyva-Porras, Macrina B. Silva-Cázares, Francisco E. Longoria-Rodríguez, Sergio A. Pérez-García, Ángel A. Vértiz-Hernández, María Z. Saavedra-Leos
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: International Journal of Polymer Science, Vol 2018 (2018)
Druh dokumentu: article
ISSN: 1687-9422
1687-9430
DOI: 10.1155/2018/5069063
Popis: Lactose is a disaccharide of importance in humans dietary, food products, and the pharmaceutical industry. From the existing isomeric forms, β-lactose is rarely found in nature. Thus, in this work, a simple methodology to obtain anhydrous β-lactose (βL) from α-lactose monohydrate (αL·H2O) is presented. The αL·H2O powder was dispersed into a basic alcoholic solution (72 hours), at controlled conditions of temperature (27, 29, 31, and 32°C), without stirring. The slurry was dried at room temperature and characterized. Fourier transform infrared spectroscopy showed the formation of βL for the samples prepared at 29 and 32°C. Raman spectroscopy confirmed this result and suggested the occurrence of crystalline βL. Rietveld refinement of the X-ray diffraction patterns was employed to identify and quantify the composition of the isomers. The samples prepared at 29 and 31°C showed the formation of pure βL, while those at 27 and 32°C showed the presence of αL·H2O and a mixture of the two isomers, respectively. The morphology of the powders was studied by scanning electron microscopy, observing the formation of irregular shape αL·H2O particles and axe-like βL particles. Clearly, with this methodology, it was possible to obtain pure, crystalline, and anhydrous βL at mild temperature.
Databáze: Directory of Open Access Journals
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