The synergic effect of alcoholic eucalyptus and nanosilver on colony count of Aspergilus Niger

Autor: Naghsh N (PhD), Doudi M (PhD), Soleymani S (MSc), Torkan S (BA)
Jazyk: perština
Rok vydání: 2012
Předmět:
Zdroj: مجله دانشگاه علوم پزشکی گرگان, Vol 14, Iss 4, Pp 89-93 (2012)
Druh dokumentu: article
ISSN: 1562-4765
2008-4080
Popis: Background and Objective: Nanosilver particles are one of functional nanotechnology filed. These nanoparticles have antibacterial and antifungal characteritic. Combination therapy is one of new and specific method for therapy of diseases in medicine and pharmacology. Some plants are useful in growth inhibition of Aspergilus Niger. This study was done to evaluate the synergic effect of alcoholic eucalyptus and nanosilver on colony count of Aspergilus Niger. Materials and Methods: In this laboratory study PAN were cultured in PDA medium. Five experimental and one control group were designed for the comparison of synergic effect of alcoholic eucalyptus and nanosilver, nanosilver 50ppm, nanosilver 12.5ppm, eucalyptus ethanolic extract 100%, eucalyptus ethanolic extract 100% mixed with nanosilver 12.5ppm, eucalyptus ethanolic extract mixed with 50ppm, and double distilled water were given to group 1, 2, 3, 4, 5 and control group respectively. Morphological, diameter, and colony numbers in various culture media were compared subsequently. The repeated number of Aspergilus Niger were 24, and they were chosen randomly. Data were analyzed using SPSS-15 and ANOVA test. Results: The Aspergilus niger colony were reduced 8 days following treatment from 200 colony in control group to 90, 75, 55 and 43 in nanosilver group in 12.5 ppm, nanosilver group in 50 ppm, nanosilver group in 50 ppm mixed with eucalyptus ethanolic extract and finally eucalyptus ethanolic extract group, respectivley (P
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