UBASH3A gene polymorphisms and expression profile in rheumatoid arthritis

Autor: Xiao-Ke Yang, Juan Liu, Shan-Yu Chen, Mu Li, Ming-Ming Zhang, Rui-Xue Leng, Hai-Feng Pan, Yuan Shen, Wen-Xue Liu, Sheng-Qian Xu, Dong-Qing Ye, Zong-Wen Shuai
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Autoimmunity, Vol 52, Iss 1, Pp 21-26 (2019)
Druh dokumentu: article
ISSN: 0891-6934
1607-842X
08916934
DOI: 10.1080/08916934.2019.1581773
Popis: Objectives: Recent evidence has demonstrated that UBASH3A play a pivotal role in multiple autoimmune diseases. In this study, we explored the association between UBASH3A gene single-nucleotide polymorphisms (SNPs) and rheumatoid arthritis (RA) in a Chinese Han population. We also comparatively evaluated the UBASH3A expression profile in peripheral blood mononuclear cells (PBMCs) from patients with RA and healthy controls. Methods: Four UBASH3A polymorphisms (rs1893592, rs11203203, rs2277798, and rs3788013) were studied in 553 patients with RA and 587 controls in a Chinese population. Genotyping was performed using the Fluidigm 192.24 Dynamic Array Integrated Fluidic Circuit (IFC). For gene expression study, UBASH3A mRNA levels of 30 RA patients and 31 healthy individuals were assessed by real-time quantitative polymerase chain reaction (RT-qPCR). Data were analyzed by SPSS 19.0 software. Results: A significant association between rs1893592 polymorphism and RA was found under all genetic models (all p.05). The mRNA expression of UBASH3A was increased in PBMCs of patients with RA when compared to healthy controls (p=.001). Conclusions: Our observations suggested that the dysregulation of UBASH3A might be associated with the pathogenesis of RA, and UBASH3A gene polymorphisms (rs1893592 and rs3788013) might contribute to RA susceptibility in Chinese Han population.
Databáze: Directory of Open Access Journals
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