| Autor: |
Li Zhang, Liang Ge, Xiao-li Ma, Jing-jing Ma, Yang Zhang, Lin-chun Sun |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Linchuang shenzangbing zazhi, Vol 24, Iss 3, Pp 216-228 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1671-2390 |
| DOI: |
10.3969/j.issn.1671-2390.2024.03.006 |
| Popis: |
Objective To explore the interaction between microRNA-23 （miR-23） and mitochondrial fission protein 1 （FIS1） in human kidney-2 （HK-2） cells and examine the effect of miR-23 on inflammatory response and oxidative stress by targeting FIS1. Methods HK-2 cells were cultured in vitro and induced by lipopolysaccharide （LPS） for modeling of sepsis. Dual luciferase reporter assay was utilized for verifying the interaction between miR-23 and FIS1. HK-2 cells were randomized into groups of miR-23 mimic/inhibitor and eukaryotic expression vector and small interfering RNA of FIS1 alone or in a combination. Cell proliferation was detected by cell counting kit-8 （CCK-8） assay and apoptosis and reactive oxygen species （ROS） were examined by flow cytometry. Malondialdehyde （MDA） was detected by MDA kit and the levels of tumor necrosis factor t （TNF-α）, interleukin （IL）-1β and IL-6/10 were measured by enzyme-linked immunosorbent assay（ELISA）. ResultsThe miR-23 mRNA in HK-2 cells induced by LPS was (0.60±0.12) folds that of control group(P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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