Utility of quantitative real time PCR in detection and monitoring of viral infections in post renal transplant recipients
| Autor: | P Shivanesan, Mukut Minz, Ranjana Walker Minz, Yashwant Kumar, Ashish Sharma, Deepesh B Kanwar, Sarabpreet Singh, H S Kohli, Shashi Anand, Ritambhra Nada |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Indian Journal of Transplantation, Vol 10, Iss 1, Pp 9-14 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2212-0017 2212-0025 |
| DOI: | 10.1016/j.ijt.2016.03.007 |
| Popis: | Background: Viral infections cause significant morbidity and mortality in post-transplant period. A highly sensitive and specific detection tool if used may help in early diagnosis and better management in these patients. The study aimed to assess the utility of quantitative real-time polymerase chain reaction (qRT-PCR) as a diagnostic and monitoring tool for viral infections in post renal transplant patients. Methods: A quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect EBV and CMV infection in 50 patients on 1st, 2nd, 3rd, and after 6 months of renal transplantation. Results: CMV infection was found in 34%, EBV in 28% of recipients, and 17% showed dual infection. Viruses were detectable after the first month of transplantation followed by symptomatic infections within first three months of follow-up, with diarrhea being the commonest symptom. These patients were also at high risk for developing other infections. Anti-thymocyte globulin (ATG) induction was a definitive risk factor for CMV/EBV infection in post operative period. Conclusion: Renal transplant patients frequently develop one or more viral infections at a time. Regular monitoring with qRT-PCR and prompt antiviral therapy with reduction in immunosuppression may be an ideal approach for management of these patients. |
| Databáze: | Directory of Open Access Journals |
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