PCR-RFLP Is a Useful Tool to Distinguish between C. Dubliniensis and C. Albicans in Cancer Patients in Iran
| Autor: | Zohreh Saltanatpouri, Tahereh Shokohi, Mohammad Bagher Hashemi Soteh, Mohammad Taghi Hedayati |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | International Journal of Hematology-Oncology and Stem Cell Research, Vol 4, Iss 2, Pp 14-18 (2010) |
| Druh dokumentu: | article |
| ISSN: | 2008-3009 2008-2207 |
| Popis: | "nIntroduction: Candida dubliniensis and C. albicans are very similar in morphology and phenotypic characteristics. Approximation of this yeast species has caused major problems in identifying these two correctly. "nMaterials and Methods: To distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was done. "nResults: PCR-RFLP of the ITS region showed different patterns between Candida dubliniensis and C. albicans after digestion with enzymes BlnI. All of the Clinical isolates were diagnosed as C .albicans. The results were confirmed by sequencing and RAPD-PCR. "nConclusion: PCR-RFLP would be a useful and applicable technique in clinical laboratories for discrimination of C. albicans and C. dubliniensis. |
| Databáze: | Directory of Open Access Journals |
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