Effects of soybean antigen proteins on intestinal permeability, 5-hydroxytryptamine levels and secretory IgA distribution in the intestine of weaned piglets

Autor: Jin Jie Wu, Cheng Ming Cao, Dan Dan Ren, Yu Zhang, Ya Nan Kou, Liang You Ma, Shi Bin Feng, Yu Li, Xi Chun Wang
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Italian Journal of Animal Science, Vol 15, Iss 1, Pp 174-180 (2016)
Druh dokumentu: article
ISSN: 1828-051X
1828051X
DOI: 10.1080/1828051X.2016.1148559
Popis: In this experiment, the effects of soybean antigen proteins on intestinal permeability, 5-hydroxytryptamine (5-HT) levels and secretory IgA (sIgA) distribution in the intestine of weaned piglets were evaluated. Thirty piglets (21 d of age) were randomly divided into three groups: control group, glycinin-sensitised group and β-conglycinin-sensitised group. Piglets in the control group were fed a basal diet, and in two sensitised groups were fed the diet with 4% glycinin or β-conglycinin. Piglets in glycinin and β-conglycinin groups were sensitised twice on 21–27 d and 32–34 d period, respectively. Blood samples were collected and analysed for D-lactic acid, diamine oxidase (DAO) and 5-HT levels on d 21, 28 and 35. At the end of the trial, five piglets per group were sacrificed and the small intestine was collected to evaluate intestinal sIgA distribution and the optical density (OD) of the relative staining positivity. The results revealed that the piglets in glycinin and β-conglycinin groups had higher D-lactic acid, DAO and 5-HT levels than those of the control group (p duodenum > proximal jejunum and distal ileum > distal jejunum. These results suggest that soybean antigen proteins induce allergic reactions, damage the intestinal mucosa, increase intestinal permeability and promote sIgA synthesis in weaned piglets.
Databáze: Directory of Open Access Journals
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