Evaluation of Genetic Variability among Three Pistacia Species Using Internal Transcribed Spacer 1 (ITS1) Marker

Autor: Amina Labdelli, Roberto De La Herrán, Francesca Resentini, Livio Trainotti, Mohammed Tahirine, Othmane Merah
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Diversity, Vol 14, Iss 12, p 1051 (2022)
Druh dokumentu: article
ISSN: 1424-2818
DOI: 10.3390/d14121051
Popis: Diversity in Pistacia has been evaluated at all molecular levels using the internal transcribed spacer 1 (ITS1) marker in three species (Pistacia atlantica subsp. atlantica; Pistacia vera and Pistacia terebinthus), and compared with other Pistacia species. Results showed that the ITS amplification and sequencing, followed by phylogenetic analyses, identify the species and confirm their classification, which revealed that it can be used as a marker. Our results suggest that ITS1 analyses might provide a simple and inexpensive approach to validate the species of samples collected from the natural population, where species identification can be difficult, especially if hybrids are present or if the season is not optimal for identifying differences in morphological traits.
Databáze: Directory of Open Access Journals
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