| Autor: |
Tae Jung Park, Jung Youn Park, Nam Su Heo, Ho Woon Choi, Soon Woo Jeong, Tae Jae Lee, Kyoung G. Lee, Seok Jae Lee |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Sensors, Vol 12, Iss 8, Pp 10810-10819 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1424-8220 |
| DOI: |
10.3390/s120810810 |
| Popis: |
Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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