| Autor: |
Madhavi Adusmilli, Nukala Sarath Babu, KS Varadarajan, Mohammed M Idris, Anant Bahadur Patel |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Magnetic Resonance Open, Vol 10, Iss , Pp 100030- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-4410 |
| DOI: |
10.1016/j.jmro.2021.100030 |
| Popis: |
Zebrafish has emerged as an excellent model organism for understanding the biology of the central nervous system. There is limited data pertaining to neurotransmitter energetics in the zebrafish brain. The present study evaluated brain energy metabolism in zebrafish by 1H-[13C]-NMR spectroscopy together with an intracardial administration of [1,6-13C2]glucose. Additionally, the impact of acute ethanol (1% in water) on brain energy metabolism was evaluated. The 13C turnover of brain amino acids from [1,6-13C2]glucose was measured in 1H-[13C]-NMR spectrum of brain tissue extract from 15 min to 90 min. The concentrations of neurometabolites in zebrafish are different than in rats and mice. There is fast incorporation of 13C labels into brain amino acids suggesting rapid glucose metabolism in the zebrafish brain. The incorporation of 13C in [4-13C]glutamate increased linearly until 30 min, and was maintained to a similar level thereafter. This is followed by [4-13C]glutamine and [3-13C]glutamate. The 13C turnover of [2–13C]GABA was much slower, and could attain ∼64% of [4-13C]glutamate labeling even at 90 min. Acute ethanol exposure significantly (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
