| Autor: |
Yulia A. Desheva, Tatiana A. Smolonogina, Galina O. Landgraf, Larisa G. Rudenko |
| Jazyk: |
English<br />Russian |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Microbiology Independent Research Journal, Vol 3, Iss 1, Pp 61-67 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2500-2236 |
| DOI: |
10.18527/2500-2236-2016-3-1-61-67 |
| Popis: |
The avian influenza viruses of H6N1subtype present a potential danger for humans. The cold-adapted ( ca ) reassortant influenza virus A/17/herring gull/Sarma/2006/887 (H6N1) was obtained in chicken embryos by the genetic reassortment based on the cold-adapted A/Leningrad/134/17/57 (H2N2) master strain. The genome composition of the obtained reassortant was analyzed by means of real-time PCR with the high resolution melting (HRM) analysis using the intercalating fluorescent dye EvaGreen. Analysis of the gene segments showed that the reassortant A/17/herring gull/Sarma/2006/887 (H6N1) contains the internal proteins coding genes (PB2, PB1, PA, NP, M, and NS) of the master donor virus and the surface antigens coding genes of the A/herring gull/Sarma/51c/2006 (H6N1) avian influenza virus. The study of the phenotypic properties showed that the virus A/17/herring gull/Sarma/2006/887 (H6N1) is temperature sensitive ( ts ), ca in chicken embryos, and attenuated in mice when administered intranasally. This reassortant can be recommended as a live influenza vaccine candidate for humans. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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