Effects of drying and storage conditions on the stability of TSH in blood spots
| Autor: | Patrícia Künzle Ribeiro Magalhães, Carlos Henrique Miranda, Fernando Crivelenti Vilar, André Schmidt, Roberta Rodrigues Bittar, Giselle Aparecida Caixe de Carvalho Paixão, Edson Zangiacomi Martinez, Léa Maria Zanini Maciel |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Archives of Endocrinology and Metabolism, Vol 62, Iss 2, Pp 201-204 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2359-4292 2359-3997 |
| DOI: | 10.20945/2359-3997000000026 |
| Popis: | ABSTRACT Objective To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening. Subjects and methods Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage. Results The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer. Conclusions Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account. |
| Databáze: | Directory of Open Access Journals |
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