Sortase-Modified Cholera Toxoids Show Specific Golgi Localization

Autor: Darren C. Machin, Daniel J. Williamson, Peter Fisher, Victoria J. Miller, Zoe L. P. Arnott, Charlotte M. E. Stevenson, Gemma C. Wildsmith, James F. Ross, Christopher W. Wasson, Andrew Macdonald, Benjamin I. Andrews, Daniel Ungar, W. Bruce Turnbull, Michael E. Webb
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Toxins, Vol 16, Iss 4, p 194 (2024)
Druh dokumentu: article
ISSN: 2072-6651
DOI: 10.3390/toxins16040194
Popis: Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase labeling approach to generate site-specific N-terminally modified variants of both the A2-B5 heterohexamer and B5 pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B5 pentamer showed an unexpectedly specific localization in the medial/trans-Golgi. This study suggests a future role for specifically labeled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labeling of lipid rafts in fixed cells.
Databáze: Directory of Open Access Journals
Nepřihlášeným uživatelům se plný text nezobrazuje