| Autor: |
Migliano Emilia, Bucher Stefania, Amantea Ada, Donati Pietro, Paolini Francesca, Venuti Aldo |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Virology Journal, Vol 8, Iss 1, p 103 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1743-422X |
| DOI: |
10.1186/1743-422X-8-103 |
| Popis: |
Abstract Background Merkel cell carcinoma (MCC) is a rare but very aggressive human malignancy of elderly or immunosuppressed patients. Clonal integration of a new human polyomavirus, the Merkel cell polyomavirus (MCPyV), has been reported in MCC patients. The main objective of the study was the detection of MCPyV and viral expression in clinical samples of Italian patients who were diagnosed MCC. Findings DNA and RNA were extracted from nine MCCs to detect the presence of MCPyV. Viral large T gene (LT1 and LT3), and viral capsid gene (VP1) were detected by polymerase chain reaction (PCR) based methods, and the amplified PCR products were subjected to direct sequencing. The presence of viral T antigen and/or viral capsid DNA sequences was demonstrated in eight of the nine MCC lesions, whereas RNA transcripts were detected in three MCCs. Conclusions These findings indicate a potential role of MCPyV in the pathogenesis of at least a subset of MCCs. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
