In silico identification of a candidate synthetic peptide (Tsgf118-43) to monitor human exposure to tsetse flies in West Africa.
| Autor: | Emilie Dama, Sylvie Cornelie, Mamadou Camara, Martin Bienvenu Somda, Anne Poinsignon, Hamidou Ilboudo, Emmanuel Elanga Ndille, Vincent Jamonneau, Philippe Solano, Franck Remoue, Zakaria Bengaly, Adrien Marie Gaston Belem, Bruno Bucheton |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2455 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1935-2727 1935-2735 |
| DOI: | 10.1371/journal.pntd.0002455 |
| Popis: | BACKGROUND: The analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. The aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three Glossina salivary proteins (Ada, Ag5 and Tsgf1) previously shown to be specifically recognized by plasma from exposed individuals. METHODOLOGY/PRINCIPAL FINDINGS: Synthetic peptides were designed by combining several linear epitope prediction methods and Blast analysis. The most specific peptides were then tested by indirect ELISA on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. Anti-Tsgf118-43 specific IgG levels were low in all three control populations (from rural Africa, urban Africa and Europe) and were significantly higher (p |
| Databáze: | Directory of Open Access Journals |
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